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4.1. Microbial Growth and Visualization of Bacteria and Fungi

chapter summary


ID Parameter Method

41_Biro Bacterial numbers Root dilution analysis

41_Blaschke Ectomycorrhiza: exploration types, hyphal network, rhizomorphs, and their response to chemical and physical properties of the soil environment Slim cases for digital recording of ectomycorrhiza formation in situ

41_Dazzo Patterns of Spatial Distribution of the Pioneer Rhizoplane Microflora Visualization of the rhizoplane microflora by computer-assisted microscopy, spatial analysis by CMEIAS image analysis

41_Dennis_a Non-destructive sampling of bacteria Non-destructive micro-scale sampling of bacteria from a root surface using a tungsten rod with a standardised sampling area controlled by a micromanipulator

41_Dennis_b Visualisation of rhizosphere bacterial colonisation patterns CCD imaging of rhizosphere colonisation patterns of Pseudomonas fluorescens SBW25 luxCDABE from soil

41_Jansa_a Isolation and culturing of arbuscular mycorrhizal fungi (AMF) Establishment and maintenance of monospecific AMF cultures

41_Jansa_b Arbuscular mycorrhizal fungal (AMF) spores Extraction of AMF spores from soil

41_Jansa_c Arbuscular mycorrhizal fungal (AMF) mycelium Assessment of AMF mycelium length density in soil

41_Jansa_d Arbuscular mycorrhizal fungal (AMF) infectivity Estimation of infectious potential of AMF in soil/inoculum

41_Jansa_e monoxenic arbuscular mycorrhizal fungal (AMF) cultures Establishment and maintenance of monoxenic AMF cultures

41_Jansa_f Root colonization by arbuscular mycorrhizal fungi (AMF) Staining of AMF colonization in roots

41_Kohler Synthesis of ectomycorrhiza Culture in perlite

41_Olsson Quantification of AM fungal signature lipids Fatty acid analysis

41_Puglisi Sterols, Phospholipid Fatty Acids (PLFAs) Characterization of soil living and dead biomass origin and structure

41_Rothballer In situ detection of bacterial gene expression in the rhizosphere translational promoter fusion

41_Schweiger_a Colonisation of roots by arbuscular mycorrhizal (AM) fungi The gridline intersection method

41_Schweiger_b Visualisation of ecto- and arbuscular mycorrhizal morphology Laser Scanning Confocal Microscopy (LSCM)

41_Takacs_a Single spore inoculum production of Arbuscular Mycorrhizal Fungi Single Spore Culture

41_Takacs_b Quality control of Arbuscular Mycorrhizal Fungi inoculum Quantification of mycorrhizal propagules density

41_Vanderleyden_a Study of bacterial growth over a long period of time BioscreenC

41_Vanderleyden_b Study of colony morphology Evaluation of colonies on plate

41_Vierheilig_a Collection of root exudates of plants colonized and non-colonized by arbuscular mycorrhizal fungi Root washing with distilled water

41_Vierheilig_b In vivo observation of arbuscular mycorrhizal structures in intact living roots Laser Scanning Confocal Microscopy (LSCM)

41_Vierheilig_c Observation of stained arbuscular mycorrhizal structures in roots Staining of arbuscular mycorrhizal fungi with ink and vinegar

41_Wallander_a Fungal biomass Ergosterol

41_Wallander_b Production of external ectomycorrhizal mycelia in the field In-growth mesh bags

> Contact: Walter Wenzel, e-mail: walter.wenzel@boku.ac.at, Phone: +43 1 47654 3125

Author: Markus Puschenreiter · last modified: 25.10.2007 20:50:12

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